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Table 1 PacBio Iso-Seq output statistics

From: Unveiling the transcriptomic complexity of Miscanthus sinensis using a combination of PacBio long read- and Illumina short read sequencing platforms

Samples

B0605

C0542

Summary of ROIs

Reads of Insert

647,252

635,350

Read bases of insert (bp)

1,453,866,187

1,413,954,821

Mean Read length of Insert (bp)

2,246

2,225

Mean Read Quality of Insert

0.98

0.98

Mean Number of Passes

11.31

10.70

Classification of ROIs

5’ primer reads

629,069

613,881

3’ primer reads

630,499

616,089

Poly-A reads

627,416

614,650

Filtered reads

15

17

NFL reads/percentage (%)

39,686

43,779

FL reads/percentage (%)

607,551

591,554

FLNC reads/percentage (%)

597,037

583,088

Average FLNC read length (bp)

2,100

2,087

CD-HIT-EST cluster of isoforms

Number of consensus isoforms

240,665

Mean length of consensus isoforms (bp)

2,535

N50 (bp)

2,863

N90 (bp)

1,710