Table 2 Analysis of MYCN gene in 4 indicators and the mean of PCR efficiency per method
From: Evaluation validation of a qPCR curve analysis method and conventional approaches
Methods | Bias(0.001) | Bias(1) | Relative error | Coefficient of variation | Precision | Resolution | −E |
|---|---|---|---|---|---|---|---|
CqMAN | 0.0000 | 0.0000 | 0.0873 | 10.86% | 0.0025 | 2.0132 | 1.8300 |
Cy0 | 0.0000 | 0.0000 | 0.0935 | 10.42% | 0.0027 | 2.1344 | NA |
LinRegPCR | 0.0008 | 0.0869 | 0.4126 | 11.32% | 0.0039 | 1.5326 | 1.8690 |
PCR-Miner | 0.0001 | 0.0054 | 0.1434 | 12.39% | 0.0057 | 2.0524 | 1.9905 |
5PSM | 0.0057 | 0.2633 | 2.5536 | 41.40% | 0.0409 | 7.0584 | 1.7462 |
DART | 0.0022 | 0.1723 | 1.0169 | 39.86% | 0.0308 | 7.1726 | 1.9047 |
FPLM | 0.0008 | 0.0617 | 0.6724 | 50.71% | 0.0609 | 7.4493 | 1.9844 |
FPK-PCR | 0.0007 | 0.1672 | 0.5012 | 29.55% | 0.0236 | 7.8181 | 2.3011 |