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Table 1 Multiple k-mer assembly statistics

From: Transcriptional expression changes during compensatory plasticity in the terminal ganglion of the adult cricket Gryllus bimaculatus

  K-mer = 21 K-mer = 25 K-mer = 27 K-mer = 30 K-mer = 32
Total # bases assembled 389,450,068 578,099,292 608,828,249 631,946,102 637,678,571
Total # assembled contigs 549,131 581,625 588,250 582,616 573,201
Total # Trinity ‘genes’ 471,672 427,875 424,223 418,137 413,346
Total # Trinity transcripts 68,117 47,537 36,867 35,325 30,180
Average contig length (bp) 709.21 993.94 1034.98 1084.67 1112.49
Median contig length (bp) 391 413 407 404 407
Maximum contig length (bp) 25,218 42,708 27,081 26,868 26,979
N50 (bp) 1072 2319 2584 2890 3027
GC count for complete assembly (%) 38.94 38.47 38.49 38.51 38.39
Overall alignment (%) 97.0 98.39 98.34 98.39 98.36
Reads mapped 1 time (%) 28.26 11.42 10.55 9.24 9.51
Reads mapped > 1 time (%) 63.30 81.10 82.02 83.48 83.07
  1. Summary metrics for five different de novo transcriptomes built with varying k-mer lengths.