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Fig. 10 | BMC Genomics

Fig. 10

From: Repertoire analysis of γδ T cells in the chicken enables functional annotation of the genomic region revealing highly variable pan-tissue TCR gamma V gene usage as well as identifying public and private repertoires

Fig. 10

Diversity of TRGV rearrangements in different tissues. Diversity indices were calculated as for the aggregate ISA Brown (n = 8 birds) chicken tissue diversity plots using all of the clones in a TRGV gene. Due to their widely varying expression levels, genes below a read count of 100 were omitted. This overcomes the issue of rarefying down to extremely low sample coverage and therefore discarding an unacceptable amount of data. 95% confidence intervals were calculated using iNEXT. A linear model was constructed from the diversity index data using a cube root transform. Error bars are 95% confidence intervals and were extracted directly from the model along with the P values. P values are comparisons between TRGV3.3 and the next most diverse TRGV gene in that tissue. Significance thresholds were as follows *** p value < 0.001, ** p value < 0.01, * p value < 0.05

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BMC Genomics

ISSN: 1471-2164

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