Fig. 3

DNA modifications of the L. rosalia mitogenome. Cytosine modifications are shown mapped to the mitogenome of L. rosalia. A) 5’methylation is shown by Nanopolish and Megalodon. B) 5′ hydroxymethylation at CpG and CH sites is shown with Megalodon. Each lane is illustrated with replicates at top and bottom from the first and second runs respectively. Hydroxymethylation in the CpG context has the highest prevalence and replication. Percentage of total modified base reads are shown from blue to red