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Table 2 Confirmation of variants. The confirmation of deletions was performed by both real-time PCR and conventional PCR with Sanger sequencing of PCR products. The verification of indel and SNP, both causing premature stop codon, used conventional PCR coupled with Sanger sequencing of PCR products. 18S real-time PCR [23] was performed to control for the presence/amount of H. meleagridis DNA

From: Complete genomes of the eukaryotic poultry parasite Histomonas meleagridis: linking sequence analysis with virulence / attenuation

strain passage number g6116vir conv. PCR g6116vir real-time PCR 18S real-time PCRa g7085vir
(conv PCR, real-time PCR)
indel (AGC Kinase) SNP (LRR)
monoxenic 2922-C6/04/DH5 α 28p + + (Ct 23.68) + (Ct16.29) + wtc wt
290p - (no Ct) + (Ct18.70) + premature stop premature stop
xenic 2922-C6/04 13p + + (27.59) + (Ct19.87) + wt wt
51p + + (Ct29.93) + (Ct17.10) + wt wt
83p + + (Ct 32.25) + (Ct21.49) + wt wt
145p - (no Ct) + (Ct19.47) + premature stop wt
237p - (no Ct) + (Ct18.05) + premature stop premature stop
292p - (no Ct) + (Ct16.23) + premature stop premature stop
xenic 2877-C3/05 22p + + (Ct25.57) + (Ct18.31) n.d.b wt wt
xenic 8175-C7/06 21p + + (Ct26.02) + (Ct18.07) n.d. wt wt
xenic 13,250-C20/10 24p + + (Ct24.83) + (Ct17.27) n.d. wt wt
316p + + (Ct22.91) + (Ct16.13) n.d. wt wt
  1. a [23]
  2. bn.d. not done
  3. cwt wild type