Fig. 5

The QuantSeq 3′ mRNA-Seq data comparison using highly degraded RNA (DV200 ≤ 30%). A; Percentage of mapped reads out of total reads by different input amounts and average fragment size of RNA. Blue, non-PCR bias-corrected reads; Orange, PCR bias-corrected reads. B; Total number of detected genes in the different inputs and average fragment size of FFPE RNA. C & D; Similarity matrix at the 10 ng and 100 ng input amounts of EF1-FFPE-30 and GT1-FFPE-30. GT1-FFPE-13, 13% of DV200; GT1-FFPE-30, 30% of DV200; EF1-FFPE-20, 20% of DV200; EF1-FFPE-30, 30% of DV200; JB1-FFPE-19, 19% of DV200; 1-FF-20, 20% of DV200