Fig. 1

Bleomycin administration protocol, lung compliance and histological evaluation of lung fibrosis. A In individual sheep (n=10), the left caudal lung segment received infusions of bleomycin, while the right caudal lung segment received the saline for controls. B Schematic diagram of bleomycin infusion protocols in sheep model. C Segmental lung compliance at the baseline and week 4. D) H & E staining was used to evaluate fibrotic score, Masson’s trichrome staining was used to evaluate % of blue staining. Ten representative randomly selected areas from each lung tissue sections, were captured under ×20 magnification for the analysis. E Fibrotic scores were expressed as median and interquartile range and (F) fibrotic fraction were expressed as means and standard errors of means. Significance was determined using Student’s t- test and Mann Whitney test denoted as follows, ** p< 0.005, **** p< 0.0001