Fig. 4

Promoter region of the UCP1 and UCP2 genes with enriched TF binding sequence and the gene-expression profile of these TFs. (A) The UCP1 promoter and enhancer region from Transcription Start Site (TSS) to − 5000 Base-Pairs (BPs); TF binding site represent nucleotide position relative to TSS; the figure only shows TFs from the 30 core-module proteins (B) The chip-seq. data show TFs that bind to the identified HIFA response elements as well as in the immediate vicinity in the UCP1 promoter and also show the SNPs coinciding with TFs binding sites. The figure was generated with the ChIPSummitDB online software. (C) Nucleotide sequence in the homologous promoter region (− 235--300 BPs) of the UCP1 and UCP2 genes (EPD database: UCP1, UCP2.2, Strand [−]), highlighting the similar region (red) and the HIF1A-HIF1B RE in a red box (EPD database). (D) The schematic figure shows TFs that bind in the promoter region (TSS - 1000 BPs) of the UCP2 gene and also shows the SNPs coinciding with TFs binding sites. The figure was created with the ChIPSummitDB online software. The chromosome region is given according to the hg19 (GRCh37) human reference genome. Red-box shows HIF1A-ARNT among the binding TFs. (E) The UCP1 promoter: -250--285 BPs show overlapping and proximal TFs binding sites with HIF1A-HIF1B response element. (F) Gene-expression profile of the TFs with Overlapping and Proximal binding site with HIF1A Response Element in UCP1 promoter