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Table 2 Nucleotide sequences of the primers were used to assay gene expression by RT-qPCR

From: Optimization of library preparation based on SMART for ultralow RNA-seq in mice brain tissues

Genes Sequences
GAPDH F: 5′-CGTCCCGTAGACAAAATGGT-3′
  R: 5′-TTGATGGCAACAATCTCCAC-3′
18S F: 5′-AAACGGCTACCACATCCAAG-3′
  R: 5′-CAATTACAGGGCCTCGAAAG-3′
Hprt1 F: 5′-CAAACTTTGCTTTCCCTGGT-3’
  R: 5′-CTGGCCTGTATCCAACACTTC-3’
  1. Note: F, forward primer; R, reversed primer