Table 2 Nucleotide sequences of the primers were used to assay gene expression by RT-qPCR
From: Optimization of library preparation based on SMART for ultralow RNA-seq in mice brain tissues
Genes | Sequences |
|---|---|
GAPDH | F: 5′-CGTCCCGTAGACAAAATGGT-3′ |
|  | R: 5′-TTGATGGCAACAATCTCCAC-3′ |
18S | F: 5′-AAACGGCTACCACATCCAAG-3′ |
|  | R: 5′-CAATTACAGGGCCTCGAAAG-3′ |
Hprt1 | F: 5′-CAAACTTTGCTTTCCCTGGT-3’ |
|  | R: 5′-CTGGCCTGTATCCAACACTTC-3’ |