Table 1 Primers used for variants validation with Sanger
Gene/ Ensembl ID | Sequence (5′- > 3′) | Annealing temperature |
|---|---|---|
ELOA/ | F: CACGGAATCTAAAGCCACAG | 57 ° C |
ENSSSCG00000025440 | R: ACTAGAGGCCAAAGCCAA | |
MYH8/ | F: CTGCCCAAGGTCATACATAC | 57 ° C |
ENSSSCG00000018005 | R: GGAATCTCCGCAGTAAAGC | |
ZNF629/ | F: CTGTGTGTGGTGTAATCCTC | 56 ° C |
ENSSSCG00000007780 | R: ACTCAGGTAGTGGAATCAGG | |
ITGAM/ | F: GTGCTGGGTTAGGGTGAAT | 56 ° C |
ENSSSCG00000007754 | R: GGGAAAGGAGTGAGGAGGA | |
CCT6A/ | F: AGCATTCATGCCTGTCTTGG | 56 °C |
ENSSSCG00000033141 | R: AACCTTGGGTCGGGTTGATT |