Fig. 4

Summary of the positions of the intragenic variants within zyg-1/ZYG-1 and their relative hatching rates. The upper image shows the zyg-1 gene arrangement along with the two 5′ UTR suppressing variants and the original it25 variant. Below, the structure of the ZYG-1 protein is outlined, with the kinase and polo domains indicated in thick blue boxes and intragenic variants affecting the protein coding region listed underneath. Variants are colour-coded in green based on their hatching ability (confirmed through testing either the mutagenized strain or the CRISPR strain). Variants that occurred twice are indicated with a plus sign, variants that occurred thrice are indicated with an asterisk, and variants that co-occurred are indicated with either a dollar sign or hashtag