Table 3 Summary of CRISPR-generated strains verifying a subset of candidate intragenic and extragenic suppressing variants
Gene | Originating Strain | Injected Strain | Variant Effects | Hatching Rate (N) | Suppressor? | Phenotype | Strain Name |
|---|---|---|---|---|---|---|---|
zyg-1 | MTG57, MTG309, and MTG406 | zyg-1(it25) | p.A385V | 48.02% (5) | Yes | Viability | MTG470 |
zyg-1 24.7 °C | MTG57, MTG309, and MTG406 | N2 | p.A385V | 94.78% (5) | N/A | Viability | MTG538 |
zyg-1 15 °C | MTG57, MTG309, and MTG406 | N2 | p.A385V | 93.13% (5) | N/A | Viability | MTG538 |
zyg-1 | MTG320 | zyg-1(it25) | p.N420K and p.H409= | 37.18% (8) | Yes | Viability | MTG498 |
zyg-1 (from 15 °C) | MTG329 | zyg-1(it25) | p.K533E | 0.19% (8) | No | Embryonic Lethality | MTG500 |
zyg-1 (from 20 °C) | MTG329 | zyg-1(it25) | p.K533E | 4.09% (5) | Yes | Viability | MTG500 |
zyg-8 | MTG329 | zyg-1(it25) | p.D31V | 0% (4) | No | Embryonic Lethality | MTG531 |
aak-2 | MTG329 | zyg-1(it25) | p.G180R | 0% (5) | No | Embryonic Lethality | MTG541 |
him-19 | MTG329 | zyg-1(it25) | p.P216S | 1.09% (5) | No | Embryonic Lethality | MTG537 |
zyg-1 | MTG398 | zyg-1(it25) | c.-4 T/A | 82.09% (7) | Yes | Viability | MTG542 |
zyg-1/+ | MTG398 | zyg-1(it25) | c.-4 T/A | 19.48% (7) | Yes | Viability | MTG542/zyg-1(it25) |