Fig. 3

The pyramid genotype has a unique transcriptome response. A. DE genes in each genotype after aphid infestation for 6 h (left) and 12 h (right) unique to each genotype or shared among different genotypes. The DE genes unique to the Rag1/2 response were designated as the “synergistic genes” set. B. Clustering heatmap showing expression patterns for the 4646 genes differentially expressed after aphid infestation in any genotype after 6 h or 12 h. Each column represents the aphid versus mock comparison for each soybean genotype and time point combination. Each row represents a gene. Genes included in this figure are differentially expressed for at least one comparison. Z-scores were calculated for each gene in all comparisons. Soybean genotypes are designated as: Sus: Susceptible, R1: Rag1, R2: Rag2, and R1R2: Rag1/2. C. Heatmap corresponding to the 1000 genes differentially expressed exclusively in the pyramid genotype at 6 h after aphid infestation, referred in the text as the synergistic response