Fig. 2
From: Universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR) for SNP genotyping
Sensitivity analysis of UPIP-qPCR. Three genotypic DNA samples of ALDH2 rs671 with concentrations from 100 ng/10 μL to 0.003 ng/10 μL showed typical S-type curves, and the appearance order of the curves was positively correlated with the decrease in concentration gradient (a, c and e). The call rates were 100% in all three genotypic samples with concentrations from 100 ng/10 μL to 0.01 ng/10 μL, but < 100% in concentrations of 0.003 ng/10μLand 0.001 ng/10 μL (b, d and f). The accuracy of all three genotypic genomic DNA concentrations was 100% (b, d and f). FAM and HEX signal curves of the same concentration in genotype GA were indicated by a gray ellipse. Each sample was detected with nine duplicates in one reaction, and the experiments were repeated four times. Bars show SD (n = 36)