Fig. 8

Multiplex primer clustering results. A The incompatibility between 14 failed target primers and 98 successfully clustered primers. B The number of false alignments between the 14 failed target primers and 98 successfully clustered primers. C The number of unspecific amplicon sites between incompatible targets and compatible group primers. D The number of new compatible targets increases as the number of unspecific amplicon site cut-off values increase when conduct multiplex compatible clustering