Fig. 11

Expression and methylation level validation of TraesCS7A02G557400 (AGO1d) in BS366 spikes under cold and control conditions. a Expression level of BS366 under cold (BS366_col) and control (BS366_con) conditions. b Methylation level of the AGO1d gene in BS366 spikes under cold and control conditions. c Bisulfite sequencing of the differentially methylated sites in the AGO1d gene in cold (1, 2) and control (3, 4) condtions. The cycle, triangle, and rectangle represent the CG, CHG and CHH contexts. The filled red, green and blue cycles represent methylated cytosine, and the unfilled cycle, triangle and rectangle represent the unmethylated cytosine sites