Fig. 12

Expression and methylation level validation of TraesCS1B02G048900 (H2A3) in BS366 between cold and control conditions. a Expression level of BS366 under cold (BS366_col) and control (BS366_con) conditions. b Methylation level of TraesCS1B02G048900 in BS366 under cold and control conditions. c Bisulfite sequencing of differentially methylated sites in the TraesCS1B02G048900 gene in cold (1, 2) and control (3, 4) condtions. The cycle, triangle, and rectangle represent the CG, CHG and CHH contexts. The filled red, green and blue cycles represent methylated and the unfilled cycle, triangle, and rectangle represent unmethylated cytosine sites