Table 5 Bacterial strains and plasmids
From: Genomewide transcriptional response of Escherichia coli O157:H7 to norepinephrine
Strain or plasmida | Genotype and description | Source or reference |
|---|---|---|
E. coli strains | Â | Â |
NADC 6564 | stx2+ and streptomycin-resistant E. coli O157:H7 | [43] |
NADC 6565 | Acid-sensitive rcsB mutant strain of NADC | [94] |
NADC 6662 | evgS evgA deletion mutant of NADC 6564 | This study |
TOP 10 | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 | Life Technologies |
|  | ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU |  |
| Â | galK rpsL (StrR) endA1 nupG | Â |
Plasmids | Â | Â |
pACYC177 | Low-copy cloning vector | New England Biolabs |
pKD46 | Recombineering vector | [106] |
pCP20 | FLP recombinase vector | [106] |
pSM779 | 4.17 kb evgS evgA operon isolated by PCR from strain NADC 6564 and cloned at SmaI site of pACYC177 | This study |