Genomewide transcriptional response of Escherichia coli O157:H7 to norepinephrine

BMC Genomics

Table 6 Primers used for PCR

Primer	Nucleotide sequence^a,d	Location^b
gadB_F^c	GTTATCTGGCGTGATGAAGAAG	2688662 - 2688683
gadB_R^c	GCGTCTAGACATCGACTGCCGTTTGCAGTG	2688757 - 2688738
gadE_F^c	TGGAGAAATTAGATGCCGAGAG	269494 - 269473
gadE_R^c	TGATACTTTCTTTGCGGCTAAC	269390 - 269411
gadX_F^c	CTCAAGGAGGAGGCATTAAATC	262426 - 262443
gadX_R^c	TTCTTATTCTGCGATAGTTGCG	262544 – 262523
hdeA_F^c	GTTATTCTTGGTGGTCTGCTTC	271319 - 271340
hdeA_R^c	GAAATCTTCACAGGTCCAGGAG	271426 - 271405
evgSA_F (deletion primer)	GAGAAGGGAGATGCTTCATTGCAAAGGGAATAATCTATGAACGGAATCAGATATCTAGCTGACTAAG	1459667 - 1459625
evgSA_R (deletion primer)	ATAGCTCCCACATTTGAACATTGTGGGAGCCACTATTTAGTTATGAATCAGATATCCTCATCTAGTTAC	1455383 - 1455426
evgSA_F (evgSA operon isolation primer)^e	CAGAATACATGAAGTTGGTGTG	1455141 - 1455162
evgSA_R (evgSA operon isolation primer)^e	CCTGTAGGATTAGTGAGAAGAC	1459865 - 1459844

^a Nucleotide sequences of primers used in this study were selected from the published genome of E. coli O157:H7 strain 6564 [43] with the accession number CP017251.1
^b Location refers to the position of primer sequence in the genome of strain 6564
^c Subscripts F and R denote forward and reverse primers, respectively
^d The underlined represents a portion of the primer sequence corresponding to the indicated location in strain 6564
^e These two primers were used for the isolation of the operon containing evgS and evgA genes and also used in PCR for confirming the deletion of evgSA operon in strain 6564

ISSN: 1471-2164