Fig. 2

Annotation and functional classification of transcripts obtained from the gonads of black rockfish. (a) Top-hit species distribution of BLASTX matches of assembled transcripts. (b) Functional annotation of assembled transcripts based on gene ontology (GO) analysis. GO analysis was performed at level 2 for the three main categories (biological process, cellular component, and molecular function). The x-axis shows the specific terms. The y-axis shows the number of transcripts for each term. (c) Pathway assignment based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The transcripts were classified into five main categories (A: cellular process, B: environmental information processing, C: genetic information processing, D: metabolism and E: organismal systems)