Fig. 5

Frequency of successful overlap positions in OLGs. 150 randomly chosen domains with a minimum length of 70 amino acids are used as a basis, resulting in 11,325 OLG pairs. In each OLG pair 30 sets of up to 50 random positions were tested against the Pfam group HMMs using the ‘biologically relevant’ threshold (5th percentile) and the ‘typical sequence’ threshold (50th percentile) for a successful overlap. While 50.3% of the pairs can be overlapped at any position and 2.5% in no position using the biological threshold only 1.9% can be overlapped at any position and 66.7% in no position using the threshold of typical sequences. The sequence threshold strongly influences the result