Fig. 6
From: Biological factors in the synthetic construction of overlapping genes
Success rates for OLG design in different reading frames as a function of threshold percentile. Each value is an average from 20 different datasets of 150 sequences with at least 70 amino acids and the error bars are equal to the standard deviation. The threshold chosen within the Pfam group has a very strong influence on success rates. The ordering of the reading frames by success rates, namely ‘-3’, ’+2’/’+3’, ‘-1’ and ‘-2’, matches the ordering by combinatorial restrictions in the standard genetic code, beginning with the least restricted frame