Fig. 4

Validation of the circularized structure of circRNAs. Seven randomly selected DE-circRNAs were confirmed by PCR experiments with divergent and convergent primers. The amplification products were tested by agarose gel electrophoresis. Each cropped gel shows the bands that were amplified with divergent and convergent primers of both circRNA and gDAN control. The back-splicing junctions of circRNA were further confirmed by Sanger sequencing