Fig. 12

Y1H assay for the interaction between PeMYB35 and the 5′ UTR/promoter of PeGT43–5. The 5′ UTR/promoter of PeGT43–5 as well as triple SMRE elements were inserted into pHIS2 vector. The reporter and effector constructs were co-transformed into yeast Y187 cells, and the transformed cells were identified by spotting serial dilutions of yeast onto selective medium SD/−Leu/−Trp (DDO) and SD/−Leu/−Trp/−His (TDO) containing 20 mmol·L^− 1 3-amino-1,2,4-triazole (3-AT). P: positive control (p53::HIS2 + pGADT7::53); N: negative control (p53::HIS2 + pGADT7::PeMYB35); Pro: promoter