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Table 9 PCR primers designed according to the mutational hotspots within four Stephania species

From: Complete chloroplast genome of Stephania tetrandra (Menispermaceae) from Zhejiang Province: insights into molecular structures, comparative genome analysis, mutational hotspots and phylogenetic relationships

Mutational hotspots

PCR primers

Expected length

trnH-psbA

F:CGCCGTAGTAAATAGGAGA

860 bp

R:TCATCAACCGYGCTAACCT

rps16

F:RATACAATAAGCAAGCTC

957 bp

R:TCCCRAAACAAGAAAACG

trnD-trnY

F:GTGCTCTGACCGATTGAACT

525 bp

R:GGCAATATGTCTACGCTGGT

trnP

F:TAGGTAGGGATGACAGGA

918 bp

R:GACCCGAACCATAGAGTA

ndhA

F:CRAATCCMAAATTAGACCA

668 bp

R:GACGCTTAGGAACACCAA

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BMC Genomics

ISSN: 1471-2164

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