Fig. 1

Study design methods from dissection to RNA isolation and data set analysis. (A) The lower reproductive tract (tissues with white text except for the ovaries labelled in red) was dissected from virgin females treated in one of three experimental conditions 1) no injection. 2) injected with saline 24 h prior to dissection 3) injected with extracts derived from male reproductive tissue (male accessory glands and seminal vesicles) 24 h prior to dissection. Total RNA isolated from these tissues was used in library construction for Illumina sequencing. (B) Multi-dimensional scaling plot of samples and replicates from this study