Fig. 3
From: InMut-finder: a software tool for insertion identification in mutagenesis using Nanopore long reads
PCR validation of insertions in soybean and M. truncatula. A, PCR results of three insertions in an Ac-Ds hybrid mutant line of soybean. Ac, primers of Ac-F and Ac-R to test the presence of Ac elements; Ds, gene-specific primer + DsL primer to test the presence of Ds insertion in the soybean genome. B, Nanopore reads are mapped to the reference of M. truncatula, and one insertion is shown exactly inside gene 1. C, The insertion of Tnt1 in gene 1 was verified by PCR and agarose gel running in six M. truncatula lines, with three alleles of wild type (lane 6), heterozygotes (lane 4 ~ 5), and homozygotes (lane 1 ~ 3)