Fig. 4

Apoptosis was evaluated using the Tunel assay, DAPI nuclear staining, and Western blotting analysis. A. Upregulation of Bax and Deptor protein, and downregulation of Stat1and Rps6ka1 protein in the livers of the LCN2-KO mice were determined by western blotting. Experiments were conducted in triplicates. Full-length blots/gels were presented in Supplementary file Fig. S1, S2, S3, S4, S5, S6 and S7. B. Representative images of the Tunel assay in the livers of the WT and LCN2-KO mice. Nucleus is blue by labeling with DAPI. Positive apoptosis cells are green. Scale bar, 200 μm. Abbreviations: Bax: apoptosis regulator BAX. Deptor: DEP domain-containing mTOR-interacting protein. Stat1: signal transducer and activator of transcription 1. Rps6ka1: Ribosomal protein S6 kinase alpha-1