Fig. 2

Scatterplot of annotated differently expressed genes and enriched signaling pathways in GWF follicles between JB and LB chickens. A MA plot of differently expressed genes in GWF follicles between JB and LB samples. Y-axis: The logarithmic value (log₂FoldChange) of the multiple of the difference in the expression level of a gene between two samples; X-axis: The negative pair value of the error detection rate (−log₁₀FDR). Red dots represent significantly up-regulated genes, blue dots indicate down-regulated genes, and grey dots imply non-differentially expressed genes (P-value adjusted for multiple testing < 0.05). JB1, GWF follicle samples of JB hens; LB1, GWF samples of LB hens. B Bubble chart of top 20 signaling pathway enrichment with KEGG pathway on the vertical axis and rich impact values on the horizontal axis. Color indicates pathway with the P-values. Bubble size represents the ratio of DEG number to the total number of genes enriched on the pathway