Fig. 5From: Transcriptome comparative analysis of ovarian follicles reveals the key genes and signaling pathways implicated in hen egg productionEvaluation and comparison of mRNA expression levels (Log2FC) of 20 candidate genes when analyzed by RNA Seq and quantitative RT-qPCR. A The candidates in the differentially expressed in GWF follicles between JB and LB hens. B The candidates in SYF follicles. C The candidates in LYF follicles. The relative expression level of each gene was quantified by the 2−ΔΔCT method and 18S rRNA was used as the internal control for normalization of the resultsBack to article page