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Fig. 8 | BMC Genomics

Fig. 8

From: Transcriptome comparative analysis of ovarian follicles reveals the key genes and signaling pathways implicated in hen egg production

Fig. 8

Silence of GABRA1 in the GCs. sh-GABRA1, GCs being transfected with GABRA1-specific shRNA; NC, scrambled shRNA; BC, no shRNA as a vehicle. A The STAR and CYP11A1 mRNA expression in the GCs was analyzed using RT-qPCR. B, C Expression of STAR and CYP11A1 proteins in the GCs with or without interference using the shRNA was analyzed by western blotting; β-actin was used as a loading control. D The CCND1, BCL-2 and CASP3 mRNA expression in the GCs was analyzed using RT-qPCR. E, F Expression of CCND1, BCL-2 and caspase-3 proteins in the GCs with or without interference using the shRNA was analyzed by western blotting

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BMC Genomics

ISSN: 1471-2164

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