Table 5 Primer pairs designed for quantitative real-time PCR analysis
Gene | Forward primer (5′ - 3′) | Reverse primer (5′ - 3′) | Accession No. | Size |
|---|---|---|---|---|
VIPR2 | ATAATGACTATGAGGACGAT | TGGATGTAGTTCCGAGTA | NM_001014970 | 154 bp |
GABRA1 | TGTGTTTTCTGCCCTCATC | ATCCTTCACCTTCTTTGGC | NM_204318 | 109 bp |
PERP1 | AGACCTTGCCCTATGTGC | GAAGTTGAACCGAAGTGTAT | NM_001277703 | 206 bp |
ZP1 | CTCCACCATTGATGTCCAGC | TCGGCGTCAGGGTAGTAGG | NM_204683 | 222 bp |
WISP1 | CCAGGATTTCCAACGACA | GACAGCCAGGCACTTCTT | NM_001024579 | 122 bp |
MC2R | TCTTCTACGCTTTGCGGTAC | ACTGGTTGGCAGTGAGGC | NM_001031515 | 245 bp |
STARD4 | AATGGACATCGTGGAAAC | AGGCTGTGGCTAGGATAG | NM_001079742 | 249 bp |
NDUFAB1 | AGGACGAGTTCGGCTTTG | TGACATCAGGGCCATCC | XM_004945263 | 157 bp |
BCL2L14 | TAAGGAACACGCAGAATC | TGTAGGAGCAGTCGCAAG | XM_040660909 | 231 bp |
LOC424014 | TGAGGATGGCTCGGTTGA | AATGGTGTAGGTGCTGATGG | XM_001232693 | 130 bp |
ADRB2 | GGAGCGACTACAACGAGG | AATCACTTCCCAAGCAACC | XM_040683024 | 255 bp |
PRLL | GCAGTAGATGAAGCGATGT | GCCATACCCAACTCCCAC | NM_001165912 | 172 bp |
HSD17B1 | CACCGCACGCACCATTCA | CGCACTCCACCAGCGTCAT | NM_204837 | 206 bp |
NCAM2 | CGGCTACAAACAGAATAGGAA | ATGAATGAAACCTTGGCAGT | XM_425540 | 124 bp |
CYP2D6 | TTACTACAACCCGCATCT | AAGGACTTCTTTCCCATC | NM_001195557 | 297 bp |
CRH | CTGGACCTGACTTTCCACCTGC | CCGCCTCACTTCCCGATGA | NM_001123031 | 116 bp |
GHRHR-LR | TGGCATTCTTCCAGTTCA | GACCCAAGTAAGCATCACAG | XM_040664932 | 167 bp |
ID4 | ACAAGCGGGTCAGCAAAGTG | TACCGGGTCGGTGTTGAGGG | NM_204282 | 179 bp |
SSTR2 | CCAACTCGGAGCCAAGAC | CAAAGGGAGCAGTCAAACA | NM_001030345 | 164 bp |
CDKN1A | GACCACGGAAGGGACTGA | AGACGGTGACGGACCACA | NM_204396 | 129 bp |
STAR | GTCCCTCGCAGACCAAGT | TCCCTACTGTTAGCCCTGA | NM_204686 | 196 bp |
CYP11A1 | GCTTTGCCTTGGAGTCTGTG | TCGGTGCTCTTGCGTTGC | NM_001001756 | 277 bp |
CCND1 | GGAGCAGAAGTGCGAAGAGG | TGGAGTTGTCGGTGTAAATG | NM_205381 | 188 bp |
BCL-2 | ATGACCGAGTACCTGAACCG | CCAAGATAAGCGCCAAGAGT | NM_205339 | 182 bp |
CASP3 | AAGAACTTCCACCGAGATACCG | GCTTAGCAACACACAAACAAAA | NM_204725 | 204 bp |
18S rRNA | ATTGGAGGGCAAGTCTGGTG | CCAGCTCGATCCCAAGATCC | AF173612 | 109 bp |