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Fig. 1 | BMC Genomics

Fig. 1

From: Caenorhabditis elegans ETR-1/CELF has broad effects on the muscle cell transcriptome, including genes that regulate translation and neuroblast migration

Fig. 1

FACS isolation of body muscle cells from wild type and etr-1(lq61) mutant L1 larvae. A Synchronized L1-stage larvae were dissociated by successive treatments with SDS-DTT and pronase to release myo-3::gfp labeled body muscle cells for isolation by FACS. B FACS scatter plot. Viable and brightly labeled myo-3::GFP marked cells (arrow) were captured for RNA extraction. Damaged cells were excluded by DAPI staining

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BMC Genomics

ISSN: 1471-2164

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