Table 1 Functions of differentially expressed genes in HCE cells (cell line and primary) infected with A. flavus CI1698
S.No | Gene symbol | Description | Function | Log2 Ratio | p value |
|---|---|---|---|---|---|
Up-regulated genes in RCB2280 cells | |||||
1 | CSF2 | Granulocyte-macrophage Colony stimulating factor 2 | growth and differentiation of hematopoietic precursor cells | 1.9681 | 0.0262 |
2 | TNFAIP3 | Tumor necrosis factor alpha-induced protein 3 | Inhibits NF-kB activity | 1.6228 | 0.0455 |
3 | LY96 | Lymphocyte antigen 96 | Innate immune responses | 1.5414 | 0.0219 |
4 | SLC2A1 | Solute carrier family 2, facilitated glucose transporter member 1 | Glucose transporter; Facilitate glucose transport to brain | 0.9197 | 0.0042 |
5 | IL3 | Interleukin 3 | Granulocyte-macrophage colony stimulating factors | 0.7921 | 0.0226 |
6 | ETS1 | Protein C-ets-1 | Transcription factor; Control expression of cytokines & chemokines | 0.5896 | 0.014 |
7 | NFIL3 | Nuclear factor interleukin-3- regulated protein | Transcription regulator; immune response | 0.581 | 0.0075 |
8 | NCAM1 | Neural cell adhesion molecule 1 | Neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites etc. | 0.5205 | 0.0279 |
9 | CDH5 | Cadherin 5 | Cell-cell adhesion; cell-cell junction assembly | 0.3546 | 0.0378 |
10 | ICAM1 | Intracellular adhesion molecule 1 | Transmembrane signaling receptor activity | 0.285 | 0.0164 |
11 | RUNX1 | Runt-related transcription factor 1 | Attenuator of NF-kB signaling | 0.2612 | 0.0487 |
12 | FN1 | Fibronectin 1 | Cell adhesion; cell motility; wound healing; cell shape maintenance | 0.236 | 0.019 |
13 | NT5E | 5’-nucleotidase | Nucleosidase activity; hydrolyze extracellular nucleotides | 0.221 | 0.0008 |
14 | PLAUR | Urokinase plasminogen activator surface receptor | Promote plasmin formation | 0.2182 | 0.0428 |
15 | NOTCH2 | Neurogenic locus notch homolog protein 2 | Regulate cell-fate determination | 0.2014 | 0.044 |
16 | CD3EAP | DNA-directed RNA polymerase I subunit RPA34 | DNA-directed 5’-3’ RNA polymerase activity; RNA binding | 0.1774 | 0.0483 |
Up-regulated genes in primary HCE cells | |||||
1 | CD6 | T-cell differentiation antigen CD6 | Regulate inflammatory responses; secretion of proinflammatory cytokines to LPS | 3.1751 | 0.0044 |
2 | CD5 | CD5 antigen like | Regulate inflammatory responses | 2.174 | 0.0093 |
3 | IL23R | Interleukin 23 R | Mediates stimulation of T-cells, NK-cells, macrophages via JAK-STAT signaling | 0.7777 | 0.0125 |
4 | PLAUR | Urokinase plasminogen activator surface receptor | Promote plasmin formation | 0.5552 | 0.0354 |
5 | ETS1 | Protein C-ets-1 | Transcription factor; Control expression of cytokines & chemokines | 0.4657 | 0.0471 |
6 | HLA-DMA | HLA class II histocompatibility antigen, DM alpha chain | Antigen processing and presentation | 0.3909 | 0.0347 |
7 | TICAM1 | TIR-domain containing adapter molecule 1 | TLR signaling | 0.3236 | 0.0134 |
8 | LGALS3 | Galectin 3 | Ligand for galactose. Innate immune response | 0.0466 | 0.048 |
Down-regulated genes in RCB2280 cells | |||||
1 | JAK1 | Tyrosine protein kinase 1 | IFN-alpha/beta/gamma signaling pathway | -0.1963 | 0.042 |
2 | SLAMF7 | SLAM family member 7 | Modulate activation and differentiation of various immune cells | -0.3343 | 0.0348 |
3 | LILRA5 | Leukocyte immunoglobulin-like receptor subfamily A member 5 | Triggering innate immune responses | -0.6356 | 0.0194 |
4 | IL19 | Interleukin 19 | Inflammatory responses | -0.7323 | 0.009 |
5 | LTB4R2 | Leukotriene B4 receptor 2 | Receptor for leukotrienes; mediates chemotaxis | -0.9056 | 0.0358 |
6 | ITGA2B | Integrin alpha-IIb | Integrin mediated signaling | -0.9215 | 0.0124 |
7 | C9 | Complement component 9 | Regulation of complement activation | -1.2485 | 0.0241 |
8 | IKZF3 | Zinc finger protein aiolos | Regulation of lymphocyte differentiation | -1.4196 | 0.0209 |
9 | LILRB2 | Leukocyte immunoglobulin-like receptor subfamily B member 2 | Down regulation of immune responses and development of tolerance | -2.5436 | 0.027 |
Down-regulated genes in primary HCE cells | |||||
1 | XCR1 | Chemokine XC receptor 1 | Calcium mediated signaling; immune and inflammatory responses | -0.5621 | 0.0201 |
2 | LILRA4 | Leukocyte immunoglobulin-like receptor subfamily A member 4 | Triggering innate immune responses | -0.7656 | 0.0259 |
3 | KLRC3 | NKG2-E type II integral membrane protein | Receptor for recognition of MHC class I molecules by NK cells and Tc cells | -0.9674 | 0.033 |
4 | JAK3 | Tyrosine protein kinase 3 | Mediates signaling event in both innate and adaptive immunity | -1.0351 | 0.0199 |