Bioinformatic comparison of Kunitz protease inhibitors in Echinococcus granulosus sensu stricto and E. multilocularis and the genes expressed in different developmental stages of E. granulosus s.s.

Table 1 Summary of physiological and biological characteristics of Kunitz protease inhibitors in E. multilocularis and E. granulosus s.s.

Species	E. multilocularis	E. granulosus s.s
KDPIs/single KDPIs	19/16	23/21
Number of amino acids	333.47	269.96
Molecular weight (Da)	37153.2	30117.02
Isoelectric points	7.44	7.84
No. of trans-domain (%)	4(21.05)	5(21.74)
No. of cysteine in Kunitz domain	5.63	5.65
No. of cysteine in the protein	24.89	18.13
Instability index	45.17	47.36
Stable protein (No/yes)	12/7	15/8
Aliphatic index	72.35	71.37
Grand average of hydropathicity	-0.22	-0.22
Signal peptides (%)	17(89.47) ^*	14(60.87)
No. of Kunitz Motifs	1.79	1.17
En-t-In(T/C)	9/2	9/4

Note: KDPIs, Kunitz domain protease inhibitors; No. of aa, number of amino acids; No. of tran-domain, percentage of containing transmembrane domains; Aver of cysteine, average of cysteine per sequence; Aliphatic indexes; GRSVY, hydropathic index; En-t-In (T/C), enzyme targeting inhibitors, trypsin inhibitors(T) or chymotrypsin inhibitors(C)
There are significant differences of signal peptides between E. multilocularis and E. granulosus s.s. KDPIs (χ² = 13.544, P < 0.01)

ISSN: 1471-2164