Table 3 Cost analysis of sickle cell and β-thal screening by nanopore sequencing. Cost estimates are based on 24 samples per library, and 8 libraries sequenced per flowcell
Item | Reactions per item | Cost per reaction | No. required per sample | Cost/test |
|---|---|---|---|---|
QiAmp DNA blood mini kit | 250 | £1.86 | 1 | £1.86 |
Enrichment Step 1 | 200 | £0.72 | 1 | £0.72 |
Enrichment Step 2 | 1000 | £0.93 | 2 | £1.86 |
Enrichment Step 3 | 50 | £9.24 | 0.04 | £0.41 |
PCR barcoding kit | 960 | £1.42 | 1 | £1.42 |
Ligation sequencing kit | 6 | £80.00 | 0.04 | £3.33 |
SpotON flowcell | 192 | £1.98 | 1 | £1.98 |
£11.57 | ||||