Fig. 6

A Synteny of the FOXO locus in amphioxus (B), chicken (G), human (H) and spotted gar (L). Genes are represented by boxes coloured and numbered by each 2R WGD family on chromosomes labelled with white text in black boxes. Each gene family has a single gene on amphioxus chromosome 9, and up to four paralogues in vertebrate genomes, e.g., the FOXO ohnologues on chicken chromosome 3 and human chromosome 6 are both adjacent to an ohnologue from the SESN (10) family. Distances are not to scale, and gene order does not exclude intervening genes. Detailed locations are listed in the supplementary information. B Maximum likelihood phylogeny of metazoan FOXO genes. Support values are IQ-TREE bootstrap support (% of 1000 replicates), MrBayes posterior probability, and MEGA Neighbor-Joining bootstrap support (% of 1000 replicates). Dashes denote missing support values for branches not present in that tree-building method. Alignment was made using MAFFT (supplementary information)