Fig. 4

Diverse and novel protein domain architectures in the expanded scallop and oyster IAP gene family. A Phylogenetic tree of IAP amino acid sequences labelled by their gene ID in C. gigas (Ostreida) (green), C. virginica (Ostreida) (blue), and M. yessoensis (Pectinida) (orange). A square node tip indicates collapsed M. yessoensis sequences for improved visualization. Node shapes indicate bootstrap support (circle = 90–100, upward triangle = 70–89, downward triangle = 50–69). When multiple transcripts from the same gene clustered together, all but one were labelled with a “---”. “---”. IAPs grouped into 21 well supported clusters. B Functional domain architecture of each transcript isoform plotted by amino acid position with domains labeled by color. Asterisk indicates transcripts where IAP repeats were only identified by Interproscan and not CDD. Shaded boxes surround each well supported cluster. C. Domain architecture type for each cluster (TI = Type I BIR, TII = Type II BIR, UBA = UBA domain, RING = RING domain, DD = Death domain, BIR* = BIR domain identified by Interproscan and not CDD). Clusters where architecture was conserved between all proteins were labelled in bold. Clusters were classified into 14 domain architecture types, 4 of which are not found in the model organisms D. melanogaster or H. sapiens