Fig. 6

Complex patterns of IAP domain architecture and gene expression across immune challenges in oysters. Comparison of IAP differential gene expression patterns across 8 transcriptomes of C. gigas and C. virginica immune challenges revealed complex expression patterns of unique and shared gene sets across experiments. C. virginica expressed more unique genes and transcripts in each experiment than C. gigas All domain architectures, including novel architectures, were significantly differentially expressed in at least one oyster, and experiments expressed unique assemblages of multiple IAP domain architectures. A Phylogenetic tree of IAP amino acid sequences labelled by their gene name in C. gigas (Ostreida) (green), C. virginica (Ostreida) (blue), and M. yessoensis (Pectinida) (orange). A square node tip indicates collapsed M. yessoensis proteins for the purpose of plotting. Node shapes indicate bootstrap support (circle = 90–100, upward triangle = 70–89, downward triangle = 50–69). Vertical bars indicate well-supported protein clusters (from Fig. 4a). Transcripts with the same amino acid sequence were collapsed by RAxML when producing the tree. Multiple transcript sequences from the same gene are named once on the lowest node and then represented by dashes (“----”). B C Heatmap of log2 fold change expression of significantly differentially expressed C. virginica (B) and C. gigas (C) IAPs in response to various immune challenge experiments (columns) plotted for each corresponding transcript in the phylogenetic tree. BAC: bacterial challenge. PM: parasitic challenge (Perkinsus marinus). OSHV1: viral challenge. Sus: susceptible oysters. Res: resistant oysters. Shaded boxes surround well supported protein clusters from (A)