Fig. 8

Apoptosis-related C. gigas differential gene expression response to immune challenge clustered mainly by susceptibility/resistance. Patterns of apoptosis pathway differential expression in response to each immune challenge in C. gigas (Ostreida) were assessed and clustered using a heatmap analysis to assess whether apoptotic responses differed between immune challenge types. This heatmap plots significantly differentially expressed apoptosis pathway transcripts with LFC > 1 in C. gigas experimental groups, colored by LFC and generated by ComplexHeatmap. Experimental treatment groups are along the X-axis, clustered by similarity of apoptosis transcript LFC. BAC: bacterial challenge. OSHV1: viral challenge. Sus: susceptible oysters. Res: resistant oysters. Apoptosis transcript IDs followed by their product name assigned by RefSeq are along the Y-axis. Total differentially expressed apoptosis-related transcripts were almost quadrupled in C. gigas compared to C. virginica (Ostreida). CGOSHV1-A susceptible and CGOSHV1-B experiments showed the most unique patterns of apoptosis expression and strongest extrinsic apoptosis pathway upregulation