Fig. 9

Apoptosis-related C. virginica differential gene expression response to immune challenge also clustered mainly by susceptibility/resistance. Patterns of apoptosis pathway differential expression in response to each immune challenge in C. virginica (Ostreida) were assessed and clustered using a heatmap analysis to assess whether the apoptotic response differed between immune challenge types. This heatmap plots significantly differentially expressed apoptosis pathway transcripts with LFC > 1 in C. virginica (Ostreida) experimental groups, colored by LFC and generated by ComplexHeatmap. Experimental treatment groups are along the X-axis, clustered by similarity of apoptosis transcript LFC. BAC: bacterial challenge. PM: parasitic challenge (Perkinsus marinus). OSHV1: viral challenge. Sus: susceptible oysters. Res: resistant oysters. Apoptosis transcript IDs followed by their product name assigned by RefSeq are along the Y-axis. CVBAC-C displayed the most unique apoptosis pathway expression, comprised mainly of extrinsic pathway transcripts. CVPMA 28d susceptible oysters also displayed strong downregulation of transcripts involved in apoptosis execution, the TLR pathway, DNA damage response, and mitochondrial dysfunction related proteins