Fig. 5

Numbers of genes having promoters (defined as 750 bp upstream to 250 bp downstream of the TSS) harboring differentially CpG-methylated regions (CpG-DMRs) for the various tissue- and injury-related comparisons. Bars indicate the total number of genes (from a total of 45,099 gene models in X. laevis v.9.1 [122]) that harbored such CpG-DMRs. Numbers above each bar indicate the fraction of such genes with DMRs >0 between the first vs. the second listed condition (hyper-methylated CpG DMR; black). CpG DMRs between uninjured regenerative vs. non-regenerative tissues (i.e., tadpole hindbrain and frog eye vs. frog hindbrain, respectively) were predominantly hypomethylated (CpG DMR < 0). With CNS injury (SCI or optic nerve crush (ONC)), CpG DMRs were predominantly hyper-methylated between injury vs. control conditions in regenerative CNS (i.e., tadpole SCI hindbrain and frog ONC eye), and hypo-methylated in non-regenerative CNS (i.e., frog SCI hindbrain)