Fig. 1

Overview of transcript assembly in S. chinensis. a. Procedure of transcript assembly for S. chinensis. Clean subreads of each size class (1 ~ 2 kb, 2 ~ 3 kb, 3 ~ 6 kb, and > 6 kb) were merged into the corresponding isoform cluster and classified as full-length (FL) and/or non-FL reads. After consensus sequence calling and quality filtration were carried out, the sequences were clustered into Iso-Seq unigenes. Unigenes derived from the Iso-Seq and Illumina de novo RNA-Seq procedures were merged after TE sequences were removed. b. The length distribution of unigenes. c. The length distribution of translated sequences (aa) predicted from unigenes. d. CEGMA evaluation. The completeness (%) of the unigenes (‘fully represented CEGs’) was assessed by CEGMA. e. Mapping of RNA-Seq samples derived from fruit and leaf tissues. In the figure, RNA-Seq data of four fruit, including Chen et al. [27] (from Jilin, China), CS_F_40 and CS_F_120 (sampled at 40 and 120 DAFs of fruit in Cheongsoon), and SB_F (Sobaeksan), and two leaf samples, including CS_L (Cheongsoon) and SB_L (Sobaeksan), were analyzed