Fig. 5

Volcano plot of differentially expressed transcripts of willow rust pathogen M. americana at 42 h post inoculation. Positive log₂ fold change (LFC) indicates up-regulation of M. americana genes when grown on the susceptible genotypes (red) while negative LFC indicates up-regulation of M. americana genes when grown on the resistant genotypes (blue). Transcripts identified by arrows were predicted to play a role in fungal infection based on in silico effector prediction software Effector P 2.0 (CDS_12834) or through sequence homology to known effectors (CDS_5062). A modified Benjamini-Hochberg adjusted p-value cutoff of < 0.1 with no log fold change cutoff was used to determine significance