Fig. 3

tRNA-derived sRNAs in rice. A Relative expression of 14-40 nt tRNA-derived reads, normalized to 4 reference genes and expressed as log2 fold change (log2FC) compared to vegetative phase roots. For details see Methods. B Classification of tRNA-derived reads based on their length and position in the tRNA gene, as described in Kumar et al. (2016) [7]. tRF-1: reads of 14-30 nt aligning in the 3′ trailer; tRF-3: reads of 14-30 nt with alignment ending in the last 3′ base of the tRNA; tRF-5: reads of 14-30 nt with alignment starting at the first 5′ base of the tRNA; 3tiR: reads of 31-40 nt with alignment ending in the last 3′ base of the tRNA; 5tiR: reads of 31-40 nt with alignment starting at the first 5′ base of the tRNA. C Frequency of occurrence of each nucleotide at the 5′ end of the different tsRNA types. In panel A and B, error bars indicate the standard deviation. Veg = vegetative phase; Gen = generative phase. Veg shoot indicates leaves and gen shoot indicates leaf blades