Fig. 5

Overexpression and knockdown of GHR gene in vitro. a Morphology of CPHs at 24 h and 48 h; bar 100 μm. b Morphology of the CPPAs at 24 h and 48 h; bar 100 μm. c, d Transfection efficiency was measured using qRT-PCR in CPHs. e, f Transfection efficiency was measured using qRT-PCR in CPPAs. g Oil Red O staining in CPPAs after induction by oleic acid-induced differentiation solution for 2 days; bar 100 μm. h Spatiotemporal expression of PPARγ gene during differentiation of CPPAs. Data are expressed as means ± SEMs, *P < 0.05, **P < 0.01, ***P < 0.001; n = 6