Table 1 Primer sequences of qRT-PCR
Genes | Primer sequences | Notes |
|---|---|---|
PPARγ | F-CTCCTTCTCCTCCCTATTT R-TTTCTTATGGATGCGACA | qRT-PCR |
FAS | F-CGCAGGCATAGCAGGAAA R-CCAAAGAAGGAGGCATCAA | qRT-PCR |
FTO | F-AGGGCAAACTTCCATACC R-ACGAGACACTGGGGTAAA | qRT-PCR |
C/EBPα | F- GACAAGAACAGCAACGAGTACC R-CCTGAAGATGCCCCGCAGAGT | qRT-PCR |
SREBP-1c | F-GGTCCGGGCCATGTTGA R-CAGGTTGGTGCGGGTGA | qRT-PCR |
A-FABP | F-ATGTGCGACCAGTTTGT R-TCACCATTGATGCTGATAG | qRT-PCR |
CYTB | F-CAGCAGACACATCCCTAGCC R-GAAGAATGAGGCGCCGTTTG | qRT-PCR |
COX1 | F-ACTACTTACCGACCGCAACC R-CCGAAACCTGGGAGGATGAG | qRT-PCR |
COX2 | F-TCGGGGTAAAAACAGACGCA R-ACTCCTGGTCGAGTGGTGAT | qRT-PCR |
COX3 | F-CCCAAGCCCATGACCAATCT R-TGGAAGGTGCTTTCTCGGAC | qRT-PCR |
ATP6 | F-TACAGCCACAATCGCCCTAC R-AGGACGAAGACGTAGGCTTG | qRT-PCR |
PGC1α | F-TCCTTTCCTCAACGCAGGTC R-TCTTGCACGTGAGGGAGAAC | qRT-PCR |
GHR | F-GCAAGTGCAGGTCACCTGAG R-CCGGACATTCTTTCCAGTCT | qRT-PCR |
GAPDH | F-CAACTTTGGCATTGTGGAGG R-CGCTGGGATGATGTTCTGG | qRT-PCR |
β-actin | F-GATATTGCTGCGCTCGTTG R-TTCAGGGTCAGGATACCTCTTT | qRT-PCR |