Table 1 Efficiency of long range PCR for the distinct fragments by the use of three commercially available enzymes
From: Long range PCR-based deep sequencing for haplotype determination in mixed HCMV infections
Amplicon | Fragment length in bases | GC content in % | total BAC-DNA as template (100 ng) | cell-culture derived DNA as template* | |||
|---|---|---|---|---|---|---|---|
TaK | Pro | Qia | TaK | TaK | |||
efficiency | efficiency | efficiency | specificity (1 × 105 copies/reaction) | sensitivity (copies/reaction) | |||
F1.1 | 7807 | 56.5 | high | low | low | low | 300 |
F1.2 | 13,807 | 58.9 | high | – | – | – | nd |
F1.3 | 18,015 | 56.5 | – | – | – | nd | nd |
F2.1 | 8278 | 57.1 | high | – | – | – | nd |
F2.2 | 13,348 | 58.8 | high | – | – | – | nd |
F2.3 | 17,861 | 56.4 | – | – | – | nd | nd |
F3 | 7705 | 53.7 | high | high | low | high | 30 |
F4 | 6671 | 48.5 | high | nd | nd | high | 30 |