Table 4a Ratio estimation of artificial mixtures used as template DNA for long range PCR
From: Long range PCR-based deep sequencing for haplotype determination in mixed HCMV infections
Artificial Mixture | HCMV-DNA | Aspired input ratio | Measured copies/ml1 | Measured ratio1 | Copy input for PCR | Measured ratio2 |
|---|---|---|---|---|---|---|
1 | Merlin | 50 | 3.90E+ 06 | 57 | 3.90E+ 04 | 44 |
TB40-BAC4-luc | 50 | 2.90E+ 06 | 43 | 2.90E+ 04 | 56 | |
2 | Merlin | 95 | 8.60E+ 06 | 94 | 8.60E+ 04 | 94 |
TB40-BAC4-luc | 5 | 4.70E+ 05 | 6 | 4.70E+ 03 | 6 | |
3 | Merlin | 5 | 4.70E+ 05 | 11 | 4.70E+ 03 | 3 |
TB40-BAC4-luc | 95 | 3.90E+ 06 | 89 | 3.90E+ 04 | 97 | |
4 | Merlin | 99 | 1.20E+ 07 | 99 | 1.20E+ 05 | 98 |
TB40-BAC4-luc | 1 | 1.30E+ 05 | 1 | 1.30E+ 03 | 2 | |
5 | Merlin | 1 | 1.90E+ 05 | 5 | 1.90E+ 03 | 1 |
TB40-BAC4-luc | 99 | 3.90E+ 06 | 95 | 3.90E+ 04 | 99 |